SCGE:18000000036 (Project)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Child Project:

SCGE:1056

Project (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000036

Description:A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.

Abbreviation:SCGE18000000036

Results found

Linked to

	Label	Description
	SCGE:1056 Project (Entity type)	SCGE study 1056
	SCGE:15000001029 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001026 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001031 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001025 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001028 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001024 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001027 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001023 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project
	SCGE:15000001032 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000036 project

SCGE:1056

Project (Entity type)

SCGE study 1056

SCGE:15000001029

Biosample (Entity type)