SCGE:18000000093 (Project)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Child Project:

SCGE:1020

Project (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000093

Description:Human kidney organoids generated from human ES and iPS cells are used to evaluate tropism of AAV2, AAV8, and AAV9 that express eGFP under CMV promoter. Organoids are exposed to AAVs at MOI 10^5 from differentiation day 21 to 28. Four culture conditions were tested: U-well suspension culture, static culture on gelbrin, low flow on a chip coated with gelbrin, and high flow on a chip coated with gelbrin. Immunohistochemistry was performed to visualize transduced cells with staining for podocytes (PODXL) and proximal tubules (LTL). AAV2/2 shows the highest transduction efficiency among three serotypes evaluated, and the GFP expression is highest in the static condition.

Abbreviation:SCGE18000000093

Results found

Linked to

	Label	Description
	SCGE:15000003418 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003403 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003409 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003415 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003412 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003405 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003404 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003414 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003406 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project
	SCGE:15000003411 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000093 project