SCGE:18000000094 (Project)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Child Project:

SCGE:1020

Project (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000094

Description:Human kidney orgnaoids generated from human ES and iPS cells are used to evaluate tropism of AAV2 that expresses eGFP under CMV promoter. Organoids are exposed to AAVs at MOI 10^5 from differentiation day 22 to 23. Four culture conditions are tested: full flow on a chip, pulsed flow on a chip, no flow on a chip, U-well suspension culture. Flow cytometry is performed to quantify the transduction efficiency in LTL+ proximal tubules. U-well culture shows the highest transduction efficiency among those four conditions.

Abbreviation:SCGE18000000094

Results found

Linked to

	Label	Description
	SCGE:15000003421 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000094 project
	SCGE:15000003423 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000094 project
	SCGE:15000003422 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000094 project
	SCGE:15000003419 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000094 project
	SCGE:1020 Project (Entity type)	SCGE study 1020
	SCGE:15000003420 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000094 project