SCGE:18000000076 (Project)

Dcc Asset:

scge cfde submission 20251016 143304.zip

Processed File (Entity type)

Dcc:

SCGE

DCC (Entity type)

Child Project:

SCGE:1079

Project (Entity type)

Persistent Id:https://scge.mcw.edu/toolkit/data/experiments/experiment/18000000076

Description:WT female mouse and homozygous male mouse containing the Gene Editing Reporter 18 (GER18) reporter driven by the EF-1 alpha promoter at the Rosa26 locus were mated. Females were super-ovulated and zygotes harvested. Zyotes were electroporated with Cas9 RNP along with two guides and were cultured 96 hours to blastocyst stage. Blastocysts were imaged for GFP fluorescence and scored for fluorescence signal. Blastocysts were then PCR amplified for the reporter allele and Sanger sequenced to determine efficiency of deleting the IVS2-654 splicing variant. ICE analysis tool from Synthego was used to score edits. NOTE: Some blasts did not PCR amplify well and it could not be determined if they had edits. Total blasts analyzed expressing the fluorescent reporter, 61. Total blast analyzed by Sanger sequencing, 54.

Abbreviation:SCGE18000000076

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	Label	Description
	SCGE:15000003448 Biosample (Entity type)	A biosample from SCGE produced as part of the SCGE:18000000076 project
	SCGE:1079 Project (Entity type)	SCGE study 1079

SCGE:15000003448

Biosample (Entity type)

A biosample from SCGE produced as part of the SCGE:18000000076 project

SCGE:1079

Project (Entity type)

SCGE study 1079

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