Description:In this experiment, we used nontargeted metabolomics to measure metabolite profiles of vitreous humor from subjects with proliferative diabetic retinopathy. Vitreous samples were collected adjacent to leaky pathological neovascular tufts that characterize PR. Amongst the top 25 most significantly dysregulated metabolites, we observed an accumulation of acylcarnitines in human neovascular PR samples compared to control subjects with epiretinal membranes.