PR002388 (Project)

Description:In this study, we constructed a mouse model of osteoporosis by performing ovariectomies and administering irisin injections. The impact of ovariectomy was assessed based on changes in body and uterine weights. Bone microstructure analysis, HE staining, MASSON staining, and Goldner staining were employed to observe alterations in bone structure. Post-irisin injection, mice showed recovery in weight and restoration of bone structure, including the formation of new bone. The presence of bone-forming factors Alkaline phosphatase (ALP), Bone Gla Protein (BGP), bone resorption factors (CTX-1, TRACP-5b), and a bone protective protein Osteoprotegerin (OPG) indicated that irisin could promote bone cell growth in bone tissue. Additionally, HE staining and immunohistochemical analysis on intestinal sections revealed that irisin restored the mucosal barrier of the intestinal wall and alleviated enlargement of intestinal epidermal cells. Analysis of IL-1β, IL-6, and TNF-α levels demonstrated irisin's role in reducing inflammation. Furthermore, sequencing and analysis of intestinal flora 16S microbial sequencing and blood metabolome in mice revealed that irisin mitigated the dysbiosis caused by osteoporosis and promoted flora growth, while enrichment analysis showed that irisin induced changes in basal metabolic levels. This study highlights the therapeutic potential of irisin in treating osteoporosis and its beneficial effects on the intestinal barrier and flora, suggesting that irisin enhances intestinal protection to foster bone tissue repair.