PR001105 (Project)

Dcc Asset:

MW submission packet 20260310.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Child Project:

PPR00001

Project (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8VT4F

Description:The right optic nerve of 1 year old female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured eyes were also collected. The tissue was dissected from euthanized fish and “flash” frozen on dry ice in Eppendorf tubes. Due to the small size of the nerves, for each category (female crush, female control, male crush, male control) n=24 the samples were pooled. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [2] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0.

Results found

Linked to

	Label	Description
	SA162372 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SA162376 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SA162379 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SA162384 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SU001802 Subject (Entity type)	A subject produced as part of the PR001105 project
	SA162386 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SA162375 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	SA162387 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project
	ST001725 Zebra Fish March 2021.zip File (Entity type)	A 4.56 GB file from Metabolomics produced from OBI:0003097 as part of the PR001105 project
	SA162373 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR001105 project