PR002655 (Project)

Description:The presented method covers all analytes involved in arginine metabolism. It is the first report for comprehensive quantification of arginine-related metabolites (ARMs) in human CSF. Furthermore, the robust and sensitive MRM based method assure analysis of samples in short run time. The in-depth understanding of perturbation of arginine related metabolites in CSF will provides useful insights on etiology of neurological disorders and progression. The present study highlighted the levels of these analytes in CSF and is benefitted with the highly sensitive multiple reaction monitoring method where simultaneous quantification of all analytes become possible in a 16 min LC-MS/MS run. The developed and validated method shows linearity in long dynamic range and provides high degree of sensitivity and selectivity for ARMs quantification in CSF. Processing of CSF samples requires very small volume (10 µL) with a short run time (16 minutes) makes it excellent for high-throughput screening of clinical samples. Comparative analysis of ARMs analytes in healthy vs clinical subject in the large cohort with neuro degenerative disorders will unearth the role of these analytes in disease progression and potentially novel biomarker for better diagnosis.