PR000841 (Project)

Dcc Asset:

MW submission packet 20260310.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Child Project:

PPR00001

Project (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8011M

Description:Objective and design: Investigate survival outcomes, and immunological and metabolomic effects of hyaluronidase (Hz) treatment during mouse models of acute inflammation and sepsis. Methods: Survival of C57Bl/6 mice was monitored after lethal challenge with lipopolysaccharide (LPS) or cecal and ligation puncture (CLP)-induced sepsis and treated with Hz or saline. Mice were also challenged with LPS and treated with Hz for leukocyte counting, cytokine quantification and determination of metabolomic profiles in the peritoneal fluid. Results: Hz treatment improved survival outcomes after lethal challenge with LPS or CLPinduced sepsis. LPS challenge promoted acute neutrophil accumulation and production of interleukin-1ß (IL-1ß) and IL-6 in the peritoneum, whereas Hz treatment suppressed neutrophil infiltration and cytokine production. We further characterized the metabolomic alterations caused by LPS challenge, which predicted activity of metabolic pathways related to fatty acids and eicosanoids. Hz treatment had a profound effect over the metabolic response, reflected by reductions of the relative levels of fatty acids. Conclusion: Collectively, these data demonstrate that Hz treatment is associated with metabolic reprogramming of pathways that sustain the inflammatory response.

Results found

Linked to

	Label	Description
	SA091287 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091260 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091293 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091296 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091294 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091297 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091284 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	ST001255 AN002084 Results.txt File (Entity type)	A 2.72 MB file from Metabolomics produced from OBI:0003097 as part of the PR000841 project
	SA091266 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project
	SA091263 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR000841 project