PR002052 (Project)

Description:This study combines these strategies to obtain in-depth untargeted chemical identification of dialysate. We target the rat dorsal and ventral striatum, given its importance in motivation, movement, and reward processing and identify 489 compounds. Despite the depth of analysis, many well-known neurochemicals, such as all neurotransmitters, were not identified in the untargeted method. These compounds could be detected using BzCl derivation in a targeted method though. By tracking isotopic BzCl feature pair detection and filtering of MS/MS spectra by known benzoyl fragment ions, we found 872 unique features in dialysate suggesting many unknown compounds remain to be identified in dialysate.