Description:Plant protection products (PPPs) can reach a range of non-target organisms including pollinators such as honeybees. Even at sublethal concentrations these can influence the host physiology, development and behaviour. In this study we investigated the structural and functional effects of sublethal, environmentally relevant concentrations of PPPs on the honeybee microbiota, gut and brain, and how these potentially affect microbiota-microbiota and microbiota-host interactions. After an acute treatment we sampled an exposure timepoint three days after exposure followed by a post-exposure timepoint ten days after exposure to determine the permanence of the observed effects. Overall, this research provides novel insights into the effects of the tested products using a combination of proteomics, metabolomics, CT imaging and bioinformatic tools.