PR002592 (Project)

Dcc Asset:

MW submission packet 20260310.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Child Project:

PPR00001

Project (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8M54Q

Description:A fundamental question in physiology is understanding how tissues adapt and alter their cellular composition in response to dietary cues. The mammalian small intestine is maintained by rapidly renewing Lgr5+ intestinal stem cells (ISCs) that respond to macronutrient changes such as fasting regimens and obesogenic diets, yet how specific amino acids control ISC function during homeostasis and injury remains unclear. Here we demonstrate that dietary cysteine, a semi-essential amino acid, enhances ISC-mediated intestinal regeneration following injury. To explore the metabolic pathways influenced by cysteine in the small intestine and colon, we performed metabolomics analysis using high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Cysteine contributes to Coenzyme A (CoA) biosynthesis in intestinal epithelial cells, which promotes expansion of intraepithelial CD8αβ+ T cells and their production of interleukin-22 (IL-22). This enhanced IL-22 signaling directly augments ISCs reparative capacity after injury.

Results found

Linked to

	Label	Description
	SA476427 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476446 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476454 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476422 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476450 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476421 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476462 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476474 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476452 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project
	SA476441 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002592 project