PR002849 (Project)

Dcc Asset:

MW submission packet 20260310.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Child Project:

PPR00001

Project (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8D84W

Description:Urinary extracellular vesicles (uEVs) offer a non-invasive source of biomarkers for kidney and urinary tract health, but progress in uEV-based metabolomics is hindered by inconsistent isolation and extraction workflows. This project established an experimentally validated, standardizable workflow for robust untargeted metabolite profiling of human uEVs. By systematically benchmarking commonly used approaches and evaluating their impact on vesicle characteristics and metabolite coverage, we identified a practical combination of conditions that maximizes recovery of biologically relevant small EVs and broadens metabolite detection. The resulting optimised workflow supports more reproducible uEV metabolomics and strengthens the foundation for EV-based biomarker discovery.

Results found

Linked to

	Label	Description
	SA537123 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537132 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537130 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537128 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537119 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537127 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537121 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537125 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537120 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project
	SA537122 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002849 project