PR002148 (Project)

Dcc Asset:

MW submission packet 20260310.zip

Processed File (Entity type)

Dcc:

Metabolomics

DCC (Entity type)

Child Project:

PPR00001

Project (Entity type)

Persistent Id:http://dx.doi.org/10.21228/M8ZR7T

Description:To assess whether branched-chain fatty acids (BCFAs) could be effectively separated and quantified using a C30 liquid chromatography column, we treated 3T3-L1 cells with a variety of lipid substrates, including odd-chain, even-chain, and branched-chain fatty acids. This initial set of experiments aimed to determine whether retention times for compound lipids shifted with the incorporation of different substrates. Despite identical lipid masses, a shift in retention time would indicate that compound lipids with monomethyl branched fatty acids could be separated. Furthermore, we engineered 3T3-L1 cells lacking branched-keto acid dehydrogenase A (Bckdha) and validated our findings using a deuterated BCFA (iso-FA 16:0[D7]). We observed incorporation of this deuterated BCFA into compound lipids, accompanied by a corresponding shift in retention time. Lipid species were analyzed via UHPLC-MS, confirming that the C30 column successfully separated monomethyl BCFA-containing lipids from their straight-chain counterparts.

Results found

Linked to

	Label	Description
	SA385770 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385772 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385780 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385779 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385777 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385776 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385773 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	SA385774 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project
	ST003502 Rawfiles.zip File (Entity type)	A 667 MB file from Metabolomics produced from OBI:0003097 as part of the PR002148 project
	SA385781 Biosample (Entity type)	A biosample from Metabolomics produced as part of the PR002148 project