Description:4DNES9IPMIAF: Live imaging of chromatin topology and transcription - parS-lambda-evePr-PP7 (control without insulator) Single particle tracking experiments (n = 15) on Drosophila embryos carrying the construct parS-lambda-evePr-PP7 (control without insulator), located at 142kb upstream to the eveMS2 allele (2R:9,836,451, dm6). Male flies with this construct were crossed with MCP::3xtagBFP/PCP::3xmKate2; parB::eGFP/+ females to produce embryos for imaging.