Description:4DNES2UUTKRG: Multiplexed FISH of AAVS1 locus in HEK293T - rTetR-KRAB (EEW25AAA-mutant) with doxycyline treatment for 1 day Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 1 day, causing recruitment of KRAB mutant (EEW25AAA) which unlike wt-KRAB is unable to silence reporter gene expression, used as negative control (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).