Description:4DNESCCHHMAL: Multiplexed FISH of AAVS1 locus in HEK293T - rTetR-KRAB (Y46A-mutant) with doxycyline treatment for 1 day Multiplexed FISH in HEK293T reporter cell line treated with doxycyline for 1 day, causing recruitment of KRAB mutant (Y46A) which is able to silence reporter gene activation but with reduced epigenetic memory compared to wt-KRAB (control and experiment relationships are described in detailed in 4DNFIWQ9QBOB supplementary file).